fish skin gelatin blocking agent  (Biotium)

Journal: Molecular Systems Biology

Article Title: Oncogenic PIK3CA corrupts growth factor signaling specificity

doi: 10.1038/s44320-024-00078-x

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Next, 75 µl of DPBS was added, followed by 19 µl of 5× fish skin gelatin blocking agent (Biotium #22010) diluted in PBS/T (PBS with 0.05% Tween-20) with 0.5% Triton X-100 (5X concentration, diluted to 0.1% once added to the DPBS).

Techniques: Knock-Out, Recombinant, Western Blot, Mass Cytometry, Sequencing, Clone Assay, Mutagenesis, Cell Culture, Membrane, Transfection, Lysis, Extraction, Protease Inhibitor, Sterility, Gel Extraction, Reverse Transcription, SYBR Green Assay, Blocking Assay, Staining, Software, Cytometry, Real-time Polymerase Chain Reaction, Microscopy

Journal: Molecular Systems Biology

Article Title: Oncogenic PIK3CA corrupts growth factor signaling specificity

doi: 10.1038/s44320-024-00078-x

Figure Lengend Snippet: Reagents and tools table

Article Snippet: 5X fish skin gelatin blocking agent , Biotium , #22010.

Techniques: Knock-Out, Recombinant, Western Blot, Mass Cytometry, Sequencing, Clone Assay, Mutagenesis, Cell Culture, Membrane, Transfection, Lysis, Extraction, Protease Inhibitor, Sterility, Gel Extraction, Reverse Transcription, SYBR Green Assay, Blocking Assay, Staining, Software, Cytometry, Real-time Polymerase Chain Reaction, Microscopy